PDBsum entry 6qil

DNA binding protein

PDB id: 6qil

Contents

Protein chains

115 a.a.

DNA/RNA

Ligands

SO4 ×14

MES

Metals

_MN ×11

Waters ×102

PDB id:

6qil

Name:

DNA binding protein

Title:

The complex structure of hsrosr-s1 (vng0258h/rosr-s1)

Structure:

DNA binding protein. Chain: a, b, c, d. Engineered: yes. DNA (28-mer). Chain: e, g. Engineered: yes. DNA (28-mer). Chain: f, h. Engineered: yes

Source:

Halobacterium salinarum (strain atcc 700922 / jcm 11081 / nrc-1). Halobacterium halobium. Organism_taxid: 64091. Strain: atcc 700922 / jcm 11081 / nrc-1. Gene: vng_0258h. Expressed in: haloferax volcanii. Expression_system_taxid: 2246. Synthetic: yes.

Resolution:

2.00Å R-factor: 0.209 R-free: 0.235

Authors:

B.Shaanan,N.Kutnowski

Key ref:

N.Kutnowski et al. (2019). Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR. Nucleic Acids Res, 47, 8860-8873. PubMed id: 31310308 DOI: 10.1093/nar/gkz604

Date:

21-Jan-19 Release date: 10-Jul-19

Protein chains

Q9HSF4  (Q9HSF4_HALSA) -  PadR family transcription regulator RosR from Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)

Seq: 116 a.a.

Struc: 115 a.a.

DNA/RNA chains

G-C-G-A-G-G-T-G-T-A-A-A-T-T-G-T-C-T-G-A-C-A-T-G-T-T-C-T 28 bases

A-G-A-A-C-A-T-G-T-C-A-G-A-C-A-A-T-T-T-A-C-A-C-C-T-C-G-C 28 bases

G-C-G-A-G-G-T-G-T-A-A-A-T-T-G-T-C-T-G-A-C-A-T-G-T-T-C-T 28 bases

A-G-A-A-C-A-T-G-T-C-A-G-A-C-A-A-T-T-T-A-C-A-C-C-T-C-G-C 28 bases

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1093/nar/gkz604

Nucleic Acids Res 47:8860-8873 (2019)

PubMed id: 31310308

Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.

N.Kutnowski, F.Shmulevich, G.Davidov, A.Shahar, D.Bar-Zvi, J.Eichler, R.Zarivach, B.Shaanan.

ABSTRACT

Interactions between proteins and DNA are crucial for all biological systems. Many studies have shown the dependence of protein-DNA interactions on the surrounding salt concentration. How these interactions are maintained in the hypersaline environments that halophiles inhabit remains puzzling. Towards solving this enigma, we identified the DNA motif recognized by the Halobactrium salinarum ROS-dependent transcription factor (hsRosR), determined the structure of several hsRosR-DNA complexes and investigated the DNA-binding process under extreme high-salt conditions. The picture that emerges from this work contributes to our understanding of the principles underlying the interplay between electrostatic interactions and salt-mediated protein-DNA interactions in an ionic environment characterized by molar salt concentrations.