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PDBsum entry 6pw6
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Viral protein/immune system
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PDB id
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6pw6
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Contents |
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439 a.a.
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126 a.a.
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49 a.a.
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References listed in PDB file
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Key reference
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Title
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Structural basis of broad HIV neutralization by a vaccine-Induced cow antibody.
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Authors
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R.L.Stanfield,
Z.T.Berndsen,
R.Huang,
D.Sok,
G.Warner,
J.L.Torres,
D.R.Burton,
A.B.Ward,
I.A.Wilson,
V.V.Smider.
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Ref.
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Sci Adv, 2020,
6,
eaba0468.
[DOI no: ]
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PubMed id
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Abstract
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Potent broadly neutralizing antibodies (bnAbs) to HIV have been very challenging
to elicit by vaccination in wild-type animals. Here, by x-ray crystallography,
cryo-electron microscopy, and site-directed mutagenesis, we structurally and
functionally elucidate the mode of binding of a potent bnAb (NC-Cow1) elicited
in cows by immunization with the HIV envelope (Env) trimer BG505 SOSIP.664. The
exceptionally long (60 residues) third complementarity-determining region of the
heavy chain (CDR H3) of NC-Cow1 forms a mini domain (knob) on an extended stalk
that navigates through the dense glycan shield on Env to target a small
footprint on the gp120 CD4 receptor binding site with no contact of the other
CDRs to the rest of the Env trimer. These findings illustrate, in molecular
detail, how an unusual vaccine-induced cow bnAb to HIV Env can neutralize with
high potency and breadth.
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