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PDBsum entry 6plh
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Immune system
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PDB id
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6plh
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References listed in PDB file
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Key reference
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Title
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Yeast- And antibody-Based tools for studying tryptophan c-Mannosylation.
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Authors
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A.John,
M.A.Järvå,
S.Shah,
R.Mao,
S.Chappaz,
R.W.Birkinshaw,
P.E.Czabotar,
A.W.Lo,
N.E.Scott,
E.D.Goddard-Borger.
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Ref.
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Nat Chem Biol, 2021,
17,
428-437.
[DOI no: ]
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PubMed id
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Abstract
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Tryptophan C-mannosylation is an unusual co-translational protein modification
performed by metazoans and apicomplexan protists. The prevalence and biological
functions of this modification are poorly understood, with progress in the field
hampered by a dearth of convenient tools for installing and detecting the
modification. Here, we engineer a yeast system to produce a diverse array of
proteins with and without tryptophan C-mannosylation and interrogate the
modification's influence on protein stability and function. This system also
enabled mutagenesis studies to identify residues of the glycosyltransferase and
its protein substrates that are crucial for catalysis. The collection of
modified proteins accrued during this work facilitated the generation and
thorough characterization of monoclonal antibodies against tryptophan
C-mannosylation. These antibodies empowered proteomic analyses of the brain
C-glycome by enriching for peptides possessing tryptophan C-mannosylation. This
study revealed many new modification sites on proteins throughout the secretory
pathway with both conventional and non-canonical consensus sequences.
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