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PDBsum entry 6p4h
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Contents |
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217 a.a.
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213 a.a.
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221 a.a.
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228 a.a.
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262 a.a.
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184 a.a.
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237 a.a.
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185 a.a.
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206 a.a.
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185 a.a.
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96 a.a.
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143 a.a.
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117 a.a.
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149 a.a.
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136 a.a.
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119 a.a.
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142 a.a.
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132 a.a.
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141 a.a.
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141 a.a.
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100 a.a.
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83 a.a.
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129 a.a.
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141 a.a.
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124 a.a.
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70 a.a.
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98 a.a.
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83 a.a.
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62 a.a.
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55 a.a.
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57 a.a.
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68 a.a.
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313 a.a.
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25 a.a.
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References listed in PDB file
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Key reference
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Title
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The israeli acute paralysis virus ires captures host ribosomes by mimicking a ribosomal state with hybrid trnas.
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Authors
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F.Acosta-Reyes,
R.Neupane,
J.Frank,
I.S.Fernández.
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Ref.
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EMBO J, 2019,
38,
e102226.
[DOI no: ]
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PubMed id
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Abstract
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Colony collapse disorder (CCD) is a multi-faceted syndrome decimating bee
populations worldwide, and a group of viruses of the widely distributed
Dicistroviridae family have been identified as a causing agent of CCD. This
family of viruses employs non-coding RNA sequences, called internal ribosomal
entry sites (IRESs), to precisely exploit the host machinery for viral protein
production. Using single-particle cryo-electron microscopy (cryo-EM), we have
characterized how the IRES of Israeli acute paralysis virus (IAPV) intergenic
region captures and redirects translating ribosomes toward viral RNA messages.
We reconstituted two in vitro reactions targeting a pre-translocation and a
post-translocation state of the IAPV-IRES in the ribosome, allowing us to
identify six structures using image processing classification methods. From
these, we reconstructed the trajectory of IAPV-IRES from the early small subunit
recruitment to the final post-translocated state in the ribosome. An early
commitment of IRES/ribosome complexes for global pre-translocation mimicry
explains the high efficiency observed for this IRES. Efforts directed toward
fighting CCD by targeting the IAPV-IRES using RNA-interference technology are
underway, and the structural framework presented here may assist in further
refining these approaches.
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