PDBsum entry 6o1h

Lyase

PDB id: 6o1h

Contents

Protein chains

268 a.a.

395 a.a.

Ligands

DMS ×8

F9F

HVK

1D0

Metals

_CL ×16

_CS ×3

Waters ×743

PDB id:

6o1h

Name:

Lyase

Title:

Tryptophan synthase q114a mutant in complex with n-(4'- trifluoromethoxybenzenesulfonyl)-2-amino-1-ethylphosphate (f9f) at the enzyme alpha-site, cesium ion at the metal coordination site, and 2-aminophenol quinonoid at the enzyme beta site

Structure:

Tryptophan synthase alpha chain. Chain: a. Engineered: yes. Tryptophan synthase beta chain. Chain: b. Engineered: yes. Mutation: yes. Other_details: beta-chain contains mutation q114a

Source:

Salmonella typhimurium. Salmonella enterica subsp. Enterica serovar typhimurium. Organism_taxid: 90371. Gene: trpa, dd95_04145. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: trpb, stm1726.

Resolution:

1.64Å R-factor: 0.156 R-free: 0.199

Authors:

E.Hilario,M.F.Dunn,L.J.Mueller,L.Fan

Key ref:

E.Hilario et al. Crystal structure of salmonella typhimurium tryptopha synthase mutant beta-Q114a with 2-({[4-(Trifluoromethoxy)phenyl]sulfonyl}amino)ethyl dihydrogen phosphate (f9f) at the alpha-Site, Cesium the metal coordination site, And [3-Hydroxy-2-Methyl-5-Phosphonooxymethyl-Pyridin-4-Yl -Serine (pls) at the beta-Site.. To be published, .

Date:

19-Feb-19 Release date: 19-Feb-20

Protein chain

P00929  (TRPA_SALTY) -  Tryptophan synthase alpha chain from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Seq: 268 a.a.

Struc: 268 a.a.

Protein chain

P0A2K1  (TRPB_SALTY) -  Tryptophan synthase beta chain from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

Seq: 397 a.a.

Struc: 395 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B: E.C.4.2.1.20 - tryptophan synthase.
• Pathway: Tryptophan Biosynthesis
• Reaction: (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O

(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O (Bound ligand (Het Group name = HVK) matches with 46.67% similarity)

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = 1D0) matches with 50.00% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site