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UniProt functional annotation for Q8TDX7 | ||
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| UniProt code: Q8TDX7. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Protein kinase which plays an important role in mitotic cell cycle progression (PubMed:17101132). Required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis (PubMed:17586473, PubMed:19414596). Phosphorylates RPS6KB1 (By similarity). Phosphorylates EML4 at 'Ser-146', promoting its dissociation from microtubules during mitosis which is required for efficient chromosome congression (PubMed:31409757). {ECO:0000250|UniProtKB:D3ZBE5, ECO:0000269|PubMed:17101132, ECO:0000269|PubMed:17586473, ECO:0000269|PubMed:19414596, ECO:0000269|PubMed:31409757}. |
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| Catalytic activity: | |
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; |
| Catalytic activity: | |
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| Activity regulation: | |
Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-97. {ECO:0000269|PubMed:19941817}. |
| Subunit: | |
Monomer (PubMed:21320329). Interacts with NEK9 (PubMed:19414596). Interacts with ANKS3; this interaction alters the subcellular distribution of NEK7 by preventing its nuclear translocation (PubMed:26188091). {ECO:0000269|PubMed:19414596, ECO:0000269|PubMed:21320329, ECO:0000269|PubMed:26188091}. |
| Subcellular location: | |
Nucleus {ECO:0000250|UniProtKB:Q9ES74}. Cytoplasm {ECO:0000269|PubMed:17586473}. Cytoplasm, cytoskeleton, spindle pole {ECO:0000269|PubMed:19414596}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:17101132, ECO:0000269|PubMed:17586473}. Note=Present at centrosome throughout the cell cycle (PubMed:17586473). Also detected at spindle midzone of the anaphase cells and eventually concentrates at the midbody (PubMed:17586473). Interaction with ANKS3 prevents its translocation to the nucleus (By similarity). {ECO:0000250|UniProtKB:Q9ES74, ECO:0000269|PubMed:17586473}. |
| Tissue specificity: | |
Highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Lower expression in ovary, prostate and kidney. No expression seen in small intestine. {ECO:0000269|PubMed:11701951}. |
| Domain: | |
Displays an autoinhibited conformation: Tyr-97 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9. {ECO:0000269|PubMed:19941817}. |
| Domain: | |
The NTE (N-terminal extension) motif is a structural component of the catalytic domain and thus contributes to activity. {ECO:0000269|PubMed:19941817}. |
| Ptm: | |
Phosphorylation at Ser-195 required for its activation. {ECO:0000269|PubMed:12840024}. |
| Similarity: | |
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.