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UniProt functional annotation for B6H6L7 | ||
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| UniProt code: B6H6L7. |
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| Organism: | |
Penicillium rubens (strain ATCC 28089 / DSM 1075 / NRRL 1951 / Wisconsin 54-1255) (Penicillium chrysogenum). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium; Penicillium chrysogenum species complex. |
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| Function: | |
Esterase that can hydrolyze the side chain of lovastatin to produce monacolin J (PubMed:28619444, PubMed:31839596). Is also able to hydrolyze the side chains of mevastatin and pravastatin, but not simvastatin (PubMed:28619444). {ECO:0000269|PubMed:28619444, ECO:0000269|PubMed:31839596}. |
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| Catalytic activity: | |
Reaction=H2O + lovastatin = (S)-2-methylbutanoate + H(+) + monacolin J; Xref=Rhea:RHEA:62748, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:40303, ChEBI:CHEBI:79034, ChEBI:CHEBI:145932; Evidence={ECO:0000269|PubMed:28619444}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62749; Evidence={ECO:0000269|PubMed:28619444}; |
| Catalytic activity: | |
Reaction=H2O + pravastatin lactone = (S)-2-methylbutanoate + H(+) + pravastatin diol lactone; Xref=Rhea:RHEA:62752, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:145931, ChEBI:CHEBI:145932, ChEBI:CHEBI:145933; Evidence={ECO:0000269|PubMed:28619444}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62753; Evidence={ECO:0000269|PubMed:28619444}; |
| Catalytic activity: | |
Reaction=H2O + mevastatin = (S)-2-methylbutanoate + compactin diol lactone + H(+); Xref=Rhea:RHEA:62744, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:34652, ChEBI:CHEBI:34848, ChEBI:CHEBI:145932; Evidence={ECO:0000269|PubMed:28619444}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:62745; Evidence={ECO:0000269|PubMed:28619444}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=39.4 uM for lovastatin (at 37 degrees Celsius) {ECO:0000269|PubMed:28619444}; KM=5.46 uM for lovastatin (at 30 degrees Celsius) {ECO:0000269|PubMed:31839596}; |
| Biotechnology: | |
PcEST can be used to directly produce monacolin J through single-step fermentation, which would be easily applied to industrial production with the existing equipment and fermentation process of lovastatin production and beneficial for the current simvastatin industry and hypercholesterolemia market. {ECO:0000269|PubMed:28619444, ECO:0000269|PubMed:31839596}. |
| Similarity: | |
Belongs to the class-A beta-lactamase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.