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PDBsum entry 6jgx
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Transcription/DNA
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PDB id
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6jgx
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References listed in PDB file
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Key reference
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Title
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Selective cadmium regulation mediated by a cooperative binding mechanism in cadr.
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Authors
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X.Liu,
Q.Hu,
J.Yang,
S.Huang,
T.Wei,
W.Chen,
Y.He,
D.Wang,
Z.Liu,
K.Wang,
J.Gan,
H.Chen.
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Ref.
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Proc Natl Acad Sci U S A, 2019,
116,
20398-20403.
[DOI no: ]
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PubMed id
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Abstract
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Detoxification of the highly toxic cadmium element is essential for the survival
of living organisms. Pseudomonas putida CadR, a MerR family
transcriptional regulator, has been reported to exhibit an ultraspecific
response to the cadmium ion. Our crystallographic and spectroscopic studies
reveal that the extra cadmium selectivity of CadR is mediated by the unexpected
cooperation of thiolate-rich site I and histidine-rich site II. Cadmium binding
in site I mediates the reorientation of protein domains and facilitates the
assembly of site II. Subsequently, site II bridge-links 2 DNA binding domains
through ligands His140/His145 in the C-terminal histidine-rich tail. With
dynamic transit between 2 conformational states, this bridge could stabilize the
regulator into an optimal conformation that is critical for enhancing the
transcriptional activity of the cadmium detoxification system. Our results
provide dynamic insight into how nature utilizes the unique cooperative binding
mechanism in multisite proteins to recognize cadmium ions specifically.
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