UniProt functional annotation for Q99436



	UniProt functional annotation for Q99436

UniProt code: Q99436.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP- dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin- independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB7 displays a trypsin-like activity. {ECO:0000269|PubMed:15244466, ECO:0000269|PubMed:27176742, ECO:0000269|PubMed:8610016}.

Catalytic activity: Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1; Evidence={ECO:0000269|PubMed:27176742};

Subunit: The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. {ECO:0000269|PubMed:25599644, ECO:0000269|PubMed:26133119, ECO:0000269|PubMed:27342858, ECO:0000269|PubMed:27428775, ECO:0000269|PubMed:27493187}.

Subunit: (Microbial infection) Interacts with HIV-1 Tat protein. {ECO:0000269|PubMed:14550573}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:12181345, ECO:0000269|PubMed:18549262}. Nucleus {ECO:0000269|PubMed:12181345, ECO:0000269|PubMed:18549262}.

Tissue specificity: Expressed at a low level in colonic mucosa. Up- regulated in colorectal cancer tissues. {ECO:0000269|PubMed:18549262}.

Similarity: Belongs to the peptidase T1B family. {ECO:0000255|PROSITE- ProRule:PRU00809}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP- dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin- independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex). Within the 20S core complex, PSMB7 displays a trypsin-like activity. {ECO:0000269\|PubMed:15244466, ECO:0000269\|PubMed:27176742, ECO:0000269\|PubMed:8610016}.


Catalytic activity:		Reaction=Cleavage of peptide bonds with very broad specificity.; EC=3.4.25.1; Evidence={ECO:0000269\|PubMed:27176742};
Subunit:		The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is a barrel-shaped complex made of 28 subunits that are arranged in four stacked rings. The two outer rings are each formed by seven alpha subunits, and the two inner rings are formed by seven beta subunits. The proteolytic activity is exerted by three beta-subunits PSMB5, PSMB6 and PSMB7. {ECO:0000269\|PubMed:25599644, ECO:0000269\|PubMed:26133119, ECO:0000269\|PubMed:27342858, ECO:0000269\|PubMed:27428775, ECO:0000269\|PubMed:27493187}.
Subunit:		(Microbial infection) Interacts with HIV-1 Tat protein. {ECO:0000269\|PubMed:14550573}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:12181345, ECO:0000269\|PubMed:18549262}. Nucleus {ECO:0000269\|PubMed:12181345, ECO:0000269\|PubMed:18549262}.
Tissue specificity:		Expressed at a low level in colonic mucosa. Up- regulated in colorectal cancer tissues. {ECO:0000269\|PubMed:18549262}.
Similarity:		Belongs to the peptidase T1B family. {ECO:0000255\|PROSITE- ProRule:PRU00809}.