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UniProt functional annotation for P21951 | ||
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| UniProt code: P21951. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Catalytic component of the DNA polymerase epsilon complex which participates in chromosomal DNA replication (PubMed:1537345). Required during synthesis of the leading DNA strands at the replication fork, binds at/or near replication origins and moves along DNA with the replication fork (PubMed:31488849). Has 3'-5' proofreading exonuclease activity that corrects errors arising during DNA replication (PubMed:12124389). {ECO:0000269|PubMed:12124389, ECO:0000269|PubMed:1537345, ECO:0000269|PubMed:31488849}. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7; Evidence={ECO:0000269|PubMed:22119860}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000269|PubMed:22119860}; Note=Binds 1 [4Fe-4S] cluster. {ECO:0000269|PubMed:22119860}; |
| Subunit: | |
DNA polymerase epsilon is a heterotetramer consisting of POL2, DPB2, DPB3 and DPB4. {ECO:0000269|PubMed:11024162}. |
| Subcellular location: | |
Nucleus. |
| Domain: | |
The DNA polymerase activity domain resides in the N-terminal half of the protein, while the C-terminus is necessary for complexing subunits B and C. {ECO:0000269|PubMed:2169349}. |
| Domain: | |
The CysA-type zinc finger is required for PCNA-binding. {ECO:0000250|UniProtKB:P15436}. |
| Domain: | |
The CysB motif binds 1 4Fe-4S cluster and is required for the formation of polymerase complexes. {ECO:0000250|UniProtKB:P15436}. |
| Miscellaneous: | |
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis. |
| Miscellaneous: | |
Present with 1970 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the DNA polymerase type-B family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.