 |
PDBsum entry 6fwc
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Tight-Binding inhibition of human monoamine oxidase b by chromone analogs: a kinetic, Crystallographic, And biological analysis.
|
|
|
Authors
|
|
J.Reis,
N.Manzella,
F.Cagide,
J.Mialet-Perez,
E.Uriarte,
A.Parini,
F.Borges,
C.Binda.
|
|
|
Ref.
|
|
J Med Chem, 2018,
61,
4203-4212.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Monoamine oxidase B (MAO-B) is a validated drug target for Parkinson's disease.
Chromone derivatives were identified as novel potent and reversible MAO-B
inhibitors, and herewith we report on a crystallographic and biochemical
analysis to investigate their inhibition mechanism. The crystal structures of
human MAO-B in complex with three chromone analogs bearing different
substituents on the exocyclic aromatic ring (determined at 1.6-1.8 Å
resolution) showed that they all bind in the active site cavity of the protein
with the chromone moiety located in front of the FAD cofactor. These inhibitors
form two hydrogen bonds with Tyr435 and Cys172 and perfectly fit the hydrophobic
flat active site of human MAO-B. This is reflected in their tight-binding
mechanism of inhibition with Ki values of 55, 17, and 31 nM for
N-(3',4'-dimethylphenyl)-4-oxo-4 H-chromene-3-carboxamide (1),
N-(3'-chlorophenyl)-4-oxo-4 H-chromene-3-carboxamide (2), and
N-(3'-fluorophenyl)-4-oxo-4 H-chromene-3-carboxamide (3), respectively. These
compounds were also 1000-fold more effective than l-deprenyl in reducing the
cellular levels of reactive oxygen species (ROS).
|
|
|
|
|
|
|
