UniProt functional annotation for A0ZZH6



	UniProt functional annotation for A0ZZH6

UniProt code: A0ZZH6.

Organism: Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a).

Taxonomy: Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.

Function: Catalyzes the reversible phosphorolysis of sucrose into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose (PubMed:14740189, PubMed:20691225). Is involved in sucrose degradation. Also displays transglucosylation activity in vitro, by transferring the glucosyl moiety of Glc1P to a broad range of monomeric sugars, such as D- and L- arabinose, D- and L-arabitol, and xylitol (PubMed:14740189). {ECO:0000269|PubMed:14740189, ECO:0000269|PubMed:20691225}.

Catalytic activity: Reaction=phosphate + sucrose = alpha-D-glucose 1-phosphate + D- fructose; Xref=Rhea:RHEA:24048, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721, ChEBI:CHEBI:43474, ChEBI:CHEBI:58601; EC=2.4.1.7; Evidence={ECO:0000269|PubMed:14740189, ECO:0000269|PubMed:20691225};

Biophysicochemical properties: Kinetic parameters: KM=6.8 mM for sucrose (at 58 degrees Celsius and pH 6.5) {ECO:0000269|PubMed:20691225}; Note=kcat is 207 sec(-1) for the phosphorolysis of sucrose (at 58 degrees Celsius and pH 6.5). {ECO:0000269|PubMed:20691225}; pH dependence: Optimum pH is 6.0-6.5 for the phosphorolysis of sucrose. {ECO:0000269|PubMed:14740189, ECO:0000269|PubMed:20691225}; Temperature dependence: Optimum temperature is 48 degrees Celsius for the phosphorolysis of sucrose (PubMed:14740189). The His-tagged enzyme shows an optimum temperature of 58 degrees Celsius (PubMed:20691225). The immobilization of the enzyme on Sepabeads EC-HFA increases thermostability, and optimum temperature is shifted to 65 degrees Celsius (PubMed:20691225). {ECO:0000269|PubMed:14740189, ECO:0000269|PubMed:20691225};

Subunit: Homodimer. {ECO:0000269|PubMed:14740189, ECO:0000269|PubMed:14756551}.

Similarity: Belongs to the glycosyl hydrolase 13 family. Sucrose phosphorylase subfamily. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bifidobacterium adolescentis (strain ATCC 15703 / DSM 20083 / NCTC 11814 / E194a).
Taxonomy:		Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium.



Function:		Catalyzes the reversible phosphorolysis of sucrose into alpha-D-glucose 1-phosphate (Glc1P) and D-fructose (PubMed:14740189, PubMed:20691225). Is involved in sucrose degradation. Also displays transglucosylation activity in vitro, by transferring the glucosyl moiety of Glc1P to a broad range of monomeric sugars, such as D- and L- arabinose, D- and L-arabitol, and xylitol (PubMed:14740189). {ECO:0000269\|PubMed:14740189, ECO:0000269\|PubMed:20691225}.


Catalytic activity:		Reaction=phosphate + sucrose = alpha-D-glucose 1-phosphate + D- fructose; Xref=Rhea:RHEA:24048, ChEBI:CHEBI:17992, ChEBI:CHEBI:37721, ChEBI:CHEBI:43474, ChEBI:CHEBI:58601; EC=2.4.1.7; Evidence={ECO:0000269\|PubMed:14740189, ECO:0000269\|PubMed:20691225};
Biophysicochemical properties:		Kinetic parameters: KM=6.8 mM for sucrose (at 58 degrees Celsius and pH 6.5) {ECO:0000269\|PubMed:20691225}; Note=kcat is 207 sec(-1) for the phosphorolysis of sucrose (at 58 degrees Celsius and pH 6.5). {ECO:0000269\|PubMed:20691225}; pH dependence: Optimum pH is 6.0-6.5 for the phosphorolysis of sucrose. {ECO:0000269\|PubMed:14740189, ECO:0000269\|PubMed:20691225}; Temperature dependence: Optimum temperature is 48 degrees Celsius for the phosphorolysis of sucrose (PubMed:14740189). The His-tagged enzyme shows an optimum temperature of 58 degrees Celsius (PubMed:20691225). The immobilization of the enzyme on Sepabeads EC-HFA increases thermostability, and optimum temperature is shifted to 65 degrees Celsius (PubMed:20691225). {ECO:0000269\|PubMed:14740189, ECO:0000269\|PubMed:20691225};
Subunit:		Homodimer. {ECO:0000269\|PubMed:14740189, ECO:0000269\|PubMed:14756551}.
Similarity:		Belongs to the glycosyl hydrolase 13 family. Sucrose phosphorylase subfamily. {ECO:0000305}.