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PDBsum entry 6faq
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DNA binding protein
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PDB id
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6faq
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References listed in PDB file
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Key reference
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Title
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The 3-D structure of vng0258h/rosr - A haloarchaeal DNA-Binding protein in its ionic shell.
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Authors
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N.Kutnowski,
H.Shmuely,
I.Dahan,
F.Shmulevich,
G.Davidov,
A.Shahar,
J.Eichler,
R.Zarivach,
B.Shaanan.
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Ref.
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J Struct Biol, 2018,
204,
191-198.
[DOI no: ]
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PubMed id
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Abstract
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Protein-DNA interactions are highly dependent on salt concentration. To gain
insight into how such interactions are maintained in the highly saline cytoplasm
of halophilic archaea, we determined the 3-D structure of VNG0258H/RosR, the
first haloarchaeal DNA-binding protein from the extreme halophilic archaeon
Halobactrium salinarum. It is a dimeric winged-helix-turn-helix (wHTH) protein
with unique features due to adaptation to the halophilic environment. As ions
are major players in DNA binding processes, particularly in halophilic
environments, we investigated the solution structure of the ionic envelope and
located anions in the first shell around the protein in the crystal using
anomalous scattering. Anions that were found to be tightly bound to residues in
the positively charged DNA-binding site would probably be released upon DNA
binding and will thus make significant contribution to the driving force of the
binding process. Unexpectedly, ions were also found in a buried internal cavity
connected to the external medium by a tunnel. Our structure lays a solid
groundwork for future structural, computational and biochemical studies on
complexes of the protein with cognate DNA sequences, with implications to
protein-DNA interactions in hyper-saline environments.
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