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PDBsum entry 6f8s
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References listed in PDB file
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Key reference
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Title
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A dual role in regulation and toxicity for the disordered n-Terminus of the toxin grat.
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Authors
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A.Talavera,
H.Tamman,
A.Ainelo,
A.Konijnenberg,
S.HadžI,
F.Sobott,
A.Garcia-Pino,
R.Hõrak,
R.Loris.
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Ref.
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Nat Commun, 2019,
10,
972.
[DOI no: ]
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PubMed id
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Abstract
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Bacterial toxin-antitoxin (TA) modules are tightly regulated to maintain growth
in favorable conditions or growth arrest during stress. A typical regulatory
strategy involves the antitoxin binding and repressing its own promoter while
the toxin often acts as a co-repressor. Here we show that Pseudomonas putida
graTA-encoded antitoxin GraA and toxin GraT differ from other TA proteins in the
sense that not the antitoxin but the toxin possesses a flexible region. GraA
auto-represses the graTA promoter: two GraA dimers bind cooperatively at
opposite sides of the operator sequence. Contrary to other TA modules, GraT is a
de-repressor of the graTA promoter as its N-terminal disordered segment prevents
the binding of the GraT2A2 complex to the operator.
Removal of this region restores operator binding and abrogates Gr aT toxicity.
GraTA represents a TA module where a flexible region in the toxin rather than in
the antitoxin controls operon expression and toxin activity.
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Secondary reference #1
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Title
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Production, Biophysical characterization and crystallization of pseudomonas putida graa and its complexes with grat and the grata operator.
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Authors
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A.Talavera,
H.Tamman,
A.Ainelo,
S.HadǽI,
A.Garcia-Pino,
R.Hõrak,
A.Konijnenberg,
R.Loris.
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Ref.
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Acta Crystallogr F Struct Biol Commun, 2017,
73,
455-462.
[DOI no: ]
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PubMed id
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