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PDBsum entry 6ebx
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Structure determination of a dimeric form of erabutoxin-B, Crystallized from a thiocyanate solution.
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Authors
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P.Saludjian,
T.Prangé,
J.Navaza,
R.Ménez,
J.P.Guilloteau,
M.Riès-Kautt,
A.Ducruix.
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Ref.
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Acta Crystallogr B, 1992,
48,
520-531.
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
95%.
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Abstract
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Erabutoxin-b, M(r) = 6861.1, a single 62 amino-acid chain folded by four
disulfide bridges, was crystallized in a new orthorhombic form by using
thiocyanate as crystallizing agent. The space group is P2(1)2(1)2(1) with a =
53.36 (4), b = 40.89 (4), c = 55.71 (5) A, V = 121533.1 A and Z = 8. X-ray
diffraction data were recorded at the LURE synchrotron facility (lambda = 1.405
A). The structure was solved by molecular replacement and shows a dimeric
association through an anti-parallel beta-sheet around the twofold
non-crystallographic axis. The two independent molecules, one SCN- ion and 97
associated water molecules were refined by molecular dynamics and annealing
techniques to R = 19.6% (10,913 Fobs, resolution 5-1.7 A). The thiocyanate ion
is located at the interface of the dimer and close to the non-crystallographic
twofold axis.
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Secondary reference #1
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Title
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Crystallization of basic proteins by ion pairing
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Authors
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M.Ries-Kautt,
A.Ducruix.
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Ref.
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TO BE PUBLISHED ...
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Secondary reference #2
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Title
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Relative effectiveness of various ions on the solubility and crystal growth of lysozyme.
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Authors
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M.M.Ries-Kautt,
A.F.Ducruix.
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Ref.
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J Biol Chem, 1989,
264,
745-748.
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PubMed id
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Secondary reference #3
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Title
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Refinement at 1.4 a resolution of a model of erabutoxin b: treatment of ordered solvent and discrete disorder.
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Authors
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J.L.Smith,
P.W.Corfield,
W.A.Hendrickson,
B.W.Low.
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Ref.
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Acta Crystallogr A, 1988,
44,
357-368.
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PubMed id
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Secondary reference #4
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Title
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Erabutoxin b. Initial protein refinement and sequence analysis at 0.140-Nm resolution.
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Authors
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P.E.Bourne,
A.Sato,
P.W.Corfield,
L.S.Rosen,
S.Birken,
B.W.Low.
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Ref.
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Eur J Biochem, 1985,
153,
521-527.
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PubMed id
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Secondary reference #5
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Title
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The three-Dimensional structure of postsynaptic snake neurotoxins. Consideration of structure and function
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Author
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B.W.Low.
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Ref.
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handb exp pharmacol, 1979,
52,
213.
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