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UniProt functional annotation for P0AG30 | ||
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| UniProt code: P0AG30. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA- dependent ATPase activity, and release of the mRNA from the DNA template. RNA-dependent NTPase which utilizes all four ribonucleoside triphosphates as substrates. {ECO:0000269|PubMed:1716628, ECO:0000269|PubMed:2461932}. |
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| Activity regulation: | |
ATPase activity is inhibited by bicyclomycin and dihydrobicyclomycin. {ECO:0000269|PubMed:7487110}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=11 uM for ATP {ECO:0000269|PubMed:7487110}; |
| Subunit: | |
Homohexamer. The homohexamer assembles into an open ring structure. {ECO:0000269|PubMed:10230401, ECO:0000269|PubMed:12859904, ECO:0000269|PubMed:15642265, ECO:0000269|PubMed:19879839, ECO:0000269|PubMed:9586995}. |
| Domain: | |
Each subunit has two RNA-binding sites, one at the surface of the hexameric ring, and one at the center of the open ring structure, where RNA helicase activity is thought to take place. |
| Similarity: | |
Belongs to the Rho family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.