|
|
||
|
|
|
|
|
UniProt functional annotation for Q38BX0 | ||
|
|
|
|
|
|
||
| UniProt code: Q38BX0. |
|
||
| Organism: | |
Trypanosoma brucei brucei (strain 927/4 GUTat10.1). |
| Taxonomy: | |
Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma. |
|
||
|
||
| Function: | |
In association with the non-catalytic regulatory subunit DHSp, catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF5A precursor protein to form the intermediate deoxyhypusine residue. Regulates protein levels of its regulatory subunit DHSp. Required for cell growth and survival. {ECO:0000269|PubMed:23525104}. |
|
||
| Catalytic activity: | |
Reaction=[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]- deoxyhypusine + propane-1,3-diamine; Xref=Rhea:RHEA:33299, Rhea:RHEA- COMP:10143, Rhea:RHEA-COMP:10144, ChEBI:CHEBI:29969, ChEBI:CHEBI:57484, ChEBI:CHEBI:57834, ChEBI:CHEBI:82657; EC=2.5.1.46; Evidence={ECO:0000269|PubMed:23525104}; |
| Cofactor: | |
Name=NAD(+); Xref=ChEBI:CHEBI:57540; Evidence={ECO:0000269|PubMed:23525104}; Note=The binding sites for NAD(+) are contained in the regulatory subunit DHSp. {ECO:0000305}; |
| Activity regulation: | |
Allosterically activated by DHSp. Inhibited by spermididine analog N1-guanyl-1,7-diamineoheptane (GC7). {ECO:0000269|PubMed:23525104}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=82 uM for NAD(+) (at 37 degrees Celsius when bound to regulatory subunit DHSp) {ECO:0000269|PubMed:23525104}; KM=0.7 uM for IF5A (at 37 degrees Celsius when bound to regulatory subunit DHSp) {ECO:0000269|PubMed:23525104}; KM=43 uM for spermidine (at 37 degrees Celsius when bound to regulatory subunit DHSp) {ECO:0000269|PubMed:23525104}; |
| Pathway: | |
Protein modification; eIF5A hypusination. {ECO:0000269|PubMed:23525104}. |
| Subunit: | |
Heterotetramer formed by a homodimer of the non-catalytic regulatory subunit DHSp and a homodimer of the catalytic subunit DHSc where DHSc appears to bind spermidine and DHSp appears to bind NAD(+). {ECO:0000269|PubMed:23525104}. |
| Subcellular location: | |
Membrane {ECO:0000255}; Single-pass membrane protein {ECO:0000255}. |
| Disruption phenotype: | |
RNAi-mediated knockdown causes cell growth arrest followed by death, loss of DHSp expression and a failure to sustain infection in mice. {ECO:0000269|PubMed:23525104}. |
| Similarity: | |
Belongs to the deoxyhypusine synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.