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UniProt functional annotation for C7C422 | ||
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| UniProt code: C7C422. |
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| Organism: | |
Klebsiella pneumoniae. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Klebsiella. |
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| Function: | |
Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. Does not confer resistance to the polymixin colistin or the fluoroquinolone ciprofloxacin. {ECO:0000269|PubMed:19770275}. |
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| Catalytic activity: | |
Reaction=a beta-lactam + H2O = a substituted beta-amino acid; Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627, ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000269|PubMed:19770275}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:22713171, ECO:0000269|PubMed:25815530}; Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000269|PubMed:22713171, ECO:0000269|PubMed:25815530}; |
| Activity regulation: | |
Inhibits by captopril, thiorphan, dimercaprol and tiopronin (PubMed:25815530). This enzyme is not susceptible to inactivation by the beta-lactamase-blocking agents clavulanic acid (PubMed:19770275). {ECO:0000269|PubMed:19770275, ECO:0000269|PubMed:25815530}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=8 uM for cefuroxime {ECO:0000269|PubMed:19770275}; KM=10 uM for cefotaxime {ECO:0000269|PubMed:19770275}; KM=10 uM for cephalothin {ECO:0000269|PubMed:19770275}; KM=12 uM for piperacillin {ECO:0000269|PubMed:19770275}; KM=16 uM for penicillin G {ECO:0000269|PubMed:19770275}; KM=22 uM for ampicillin {ECO:0000269|PubMed:19770275}; KM=49 uM for cefoxitin {ECO:0000269|PubMed:19770275}; KM=49 uM for meropenem {ECO:0000269|PubMed:19770275}; KM=77 uM for cefepime {ECO:0000269|PubMed:19770275}; KM=94 uM for imipenem {ECO:0000269|PubMed:19770275}; KM=181 uM for ceftazidime {ECO:0000269|PubMed:19770275}; Note=No activity detected against the monobactam aztreonam. {ECO:0000269|PubMed:19770275}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:19770275, ECO:0000269|PubMed:22713171, ECO:0000269|PubMed:25815530}. |
| Subcellular location: | |
Periplasm {ECO:0000305}. |
| Miscellaneous: | |
Enterobacteriaceae carrying this gene (identified by PCR) have been isolated in Sweden, India, Pakistan, Bangladesh, England, Scotland, Northern Ireland, Australia and the USA. The organisms they were identified in were K.pneumoniae, Enterobacter cloacae, E.coli, Proteus spp, Citrobacter freundii, Morganella morganii, Providencia spp and Klebsille oxytoca. In India most isolates were from community-acquired infections rather than hospital-acquired infections, indicating the gene is widespread in the environment. {ECO:0000269|PubMed:20577157, ECO:0000269|PubMed:20705517}. |
| Similarity: | |
Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.