UniProt functional annotation for P36217



	UniProt functional annotation for P36217

UniProt code: P36217.

Organism: Hypocrea jecorina (strain ATCC 56765 / BCRC 32924 / NRRL 11460 / Rut C-30) (Trichoderma reesei).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.

Function: Glycoside hydrolase involved in the hydrolysis of xylan, a major plant cell wall hemicellulose made up of 1,4-beta-linked D- xylopyranose residues. Catalyzes the endohydrolysis of the main-chain 1,4-beta-glycosidic bonds connecting the xylose subunits yielding various xylooligosaccharides and xylose (PubMed:1369024, Ref.5). The catalysis proceeds by a double-displacement reaction mechanism with a putative covalent glycosyl-enzyme intermediate, with retention of the anomeric configuration (PubMed:7988708). Produces xylobiose and xylose as the main degradation products (PubMed:19556747). {ECO:0000269|PubMed:1369024, ECO:0000269|PubMed:19556747, ECO:0000269|PubMed:7988708, ECO:0000269|Ref.5}.

Catalytic activity: Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269|PubMed:1369024, ECO:0000269|Ref.5};

Biophysicochemical properties: Kinetic parameters: KM=0.14 mg/ml for beechwood (unsubstituted) xylan {ECO:0000269|PubMed:1369024}; KM=13.8 mg/ml for birchwood xylan {ECO:0000269|PubMed:17416973}; KM=3.0 mg/ml for acetylated glucuronoxylan {ECO:0000269|Ref.5}; KM=3.8 mg/ml for deacetylated glucuronoxylan {ECO:0000269|Ref.5}; KM=6.8 mg/ml for unsubstituted xylan {ECO:0000269|Ref.5}; KM=73 uM for xylohexaose {ECO:0000269|PubMed:17475200}; KM=136 uM for xylopentaose {ECO:0000269|PubMed:17475200}; Vmax=1600 umol/min/mg enzyme for beechwood xylan {ECO:0000269|PubMed:1369024}; Vmax=336 umol/min/mg enzyme for birchwood xylan {ECO:0000269|PubMed:17416973}; Note=kcat is 68 sec(-1) with xylohexaose, 50.3 sec(-1) with xylopentaose, 0.162 sec(-1) with xylotetraose and 0.045 sec(-1) with xylotriose as substrate. {ECO:0000269|PubMed:17475200}; pH dependence: Optimum pH is 4.5-5.5 (PubMed:1369024). Stable from pH 3.0 to 8.5 at room temperature and from pH 4.0 to 7.5 at 40 degrees Celsius (Ref.5). {ECO:0000269|PubMed:1369024, ECO:0000269|Ref.5};

Pathway: Glycan degradation; xylan degradation. {ECO:0000255|PROSITE- ProRule:PRU01097}.

Subcellular location: Secreted {ECO:0000269|Ref.5}.

Induction: Induced by D-xylose, dependent on the cellulase and xylanase regulator xyr1. Repressed by glucose through negative regulation by the crabon catabolite repressor cre1. {ECO:0000250|UniProtKB:G0RUP7}.

Similarity: Belongs to the glycosyl hydrolase 11 (cellulase G) family. {ECO:0000305}.

Sequence caution: Sequence=CAA49293.1; Type=Frameshift; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Hypocrea jecorina (strain ATCC 56765 / BCRC 32924 / NRRL 11460 / Rut C-30) (Trichoderma reesei).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.



Function:		Glycoside hydrolase involved in the hydrolysis of xylan, a major plant cell wall hemicellulose made up of 1,4-beta-linked D- xylopyranose residues. Catalyzes the endohydrolysis of the main-chain 1,4-beta-glycosidic bonds connecting the xylose subunits yielding various xylooligosaccharides and xylose (PubMed:1369024, Ref.5). The catalysis proceeds by a double-displacement reaction mechanism with a putative covalent glycosyl-enzyme intermediate, with retention of the anomeric configuration (PubMed:7988708). Produces xylobiose and xylose as the main degradation products (PubMed:19556747). {ECO:0000269\|PubMed:1369024, ECO:0000269\|PubMed:19556747, ECO:0000269\|PubMed:7988708, ECO:0000269\|Ref.5}.


Catalytic activity:		Reaction=Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.; EC=3.2.1.8; Evidence={ECO:0000269\|PubMed:1369024, ECO:0000269\|Ref.5};
Biophysicochemical properties:		Kinetic parameters: KM=0.14 mg/ml for beechwood (unsubstituted) xylan {ECO:0000269\|PubMed:1369024}; KM=13.8 mg/ml for birchwood xylan {ECO:0000269\|PubMed:17416973}; KM=3.0 mg/ml for acetylated glucuronoxylan {ECO:0000269\|Ref.5}; KM=3.8 mg/ml for deacetylated glucuronoxylan {ECO:0000269\|Ref.5}; KM=6.8 mg/ml for unsubstituted xylan {ECO:0000269\|Ref.5}; KM=73 uM for xylohexaose {ECO:0000269\|PubMed:17475200}; KM=136 uM for xylopentaose {ECO:0000269\|PubMed:17475200}; Vmax=1600 umol/min/mg enzyme for beechwood xylan {ECO:0000269\|PubMed:1369024}; Vmax=336 umol/min/mg enzyme for birchwood xylan {ECO:0000269\|PubMed:17416973}; Note=kcat is 68 sec(-1) with xylohexaose, 50.3 sec(-1) with xylopentaose, 0.162 sec(-1) with xylotetraose and 0.045 sec(-1) with xylotriose as substrate. {ECO:0000269\|PubMed:17475200}; pH dependence: Optimum pH is 4.5-5.5 (PubMed:1369024). Stable from pH 3.0 to 8.5 at room temperature and from pH 4.0 to 7.5 at 40 degrees Celsius (Ref.5). {ECO:0000269\|PubMed:1369024, ECO:0000269\|Ref.5};
Pathway:		Glycan degradation; xylan degradation. {ECO:0000255\|PROSITE- ProRule:PRU01097}.
Subcellular location:		Secreted {ECO:0000269\|Ref.5}.
Induction:		Induced by D-xylose, dependent on the cellulase and xylanase regulator xyr1. Repressed by glucose through negative regulation by the crabon catabolite repressor cre1. {ECO:0000250\|UniProtKB:G0RUP7}.
Similarity:		Belongs to the glycosyl hydrolase 11 (cellulase G) family. {ECO:0000305}.
Sequence caution:		Sequence=CAA49293.1; Type=Frameshift; Evidence={ECO:0000305};