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PDBsum entry 5z4z
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DNA binding protein
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PDB id
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5z4z
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References listed in PDB file
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Key reference
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Title
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Molecular insights into the master regulator cysb-Mediated bacterial virulence in pseudomonas aeruginosa.
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Authors
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Y.Song,
C.Yang,
G.Chen,
Y.Zhang,
Z.Seng,
Z.Cai,
C.Zhang,
L.Yang,
J.Gan,
H.Liang.
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Ref.
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Mol Microbiol, 2019,
111,
1195-1210.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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Pseudomonas aeruginosa is a major pathogen that causes serious acute and chronic
infections in humans. The type III secretion system (T3SS) is an important
virulence factor that plays essential roles in acute infections. However, the
regulatory mechanisms of T3SS are not fully understood. In this study, we found
that the deletion of cysB reduced the T3SS gene expression and swarming motility
but enhanced biofilm formation. In a mouse acute pneumonia model, mutation of
cysB decreased the average bacterial load compared to that of the wild-type
strain. Further experiments demonstrated that CysB contributed to the reduced
T3SS gene expression and bacterial pathogenesis by directly regulating the
sensor kinase RetS. We also performed crystallographic studies of PaCysB. The
overall fold of PaCysB NTD domain is similar to other LysR superfamily proteins
and structural superposition revealed one possible DNA-binding model for PaCysB.
Structural comparison also revealed great flexibility of the PaCysB RD domain,
which may play an important role in bending and transcriptional regulation of
target DNA. Taken together, these results expand our current understanding of
the complex regulatory networks of T3SS and RetS pathways. The crystal structure
of CysB provides new insights for studying the function of its homologs in other
bacterial species.
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