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UniProt functional annotation for Q03532 | ||
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| UniProt code: Q03532. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
ATP-dependent RNA helicase involved in 40S ribosomal subunit biogenesis. Required for the processing and cleavage of 35S pre-rRNA at sites A0, A1, and A2, leading to mature 18S rRNA. {ECO:0000269|PubMed:15049817, ECO:0000269|PubMed:15242642, ECO:0000269|PubMed:15718299}. |
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| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=450 uM for ATP {ECO:0000269|PubMed:15718299}; pH dependence: Optimum pH is 6.5. Active from pH 5 to 8. {ECO:0000269|PubMed:15718299}; |
| Subunit: | |
Interacts with RRP1. Associates in the nucleolus with the 60S and pre-60S ribosomal subunits. It has also been isolated with the nuclear pore complex. {ECO:0000269|PubMed:15100437, ECO:0000269|PubMed:15769876}. |
| Subcellular location: | |
Nucleus, nucleolus {ECO:0000269|PubMed:10684247, ECO:0000269|PubMed:15049817}. |
| Domain: | |
The Q motif is unique to and characteristic of the DEAD box family of RNA helicases and controls ATP binding and hydrolysis. |
| Ptm: | |
Phosphorylated by CDK1. {ECO:0000305|PubMed:14574415}. |
| Miscellaneous: | |
Transcription depends partially on SET1. |
| Similarity: | |
Belongs to the DEAD box helicase family. DDX18/HAS1 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.