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UniProt functional annotation for Q8TGZ7 | ||
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| UniProt code: Q8TGZ7. |
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| Organism: | |
Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938). |
| Taxonomy: | |
Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae; Methanopyrus. |
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| Function: | |
Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp. {ECO:0000255|HAMAP- Rule:MF_01833}. |
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| Catalytic activity: | |
Reaction=PretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'- half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.; EC=4.6.1.16; Evidence={ECO:0000255|HAMAP-Rule:MF_01833}; |
| Subunit: | |
Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers. {ECO:0000255|HAMAP-Rule:MF_01833}. |
| Similarity: | |
Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily. {ECO:0000255|HAMAP-Rule:MF_01833}. |
Annotations taken from UniProtKB at the EBI.