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UniProt functional annotation for P9WP23 | ||
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| UniProt code: P9WP23. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate (Probable). Can use both NADH and NADPH as a reductant, with NADH being 6-fold as effective as NADPH. {ECO:0000255|HAMAP-Rule:MF_00102, ECO:0000269|PubMed:12962488, ECO:0000305}. |
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| Catalytic activity: | |
Reaction=(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NAD(+) = (2S,4S)-4- hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADH; Xref=Rhea:RHEA:35323, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16845, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:67139; EC=1.17.1.8; Evidence={ECO:0000255|HAMAP- Rule:MF_00102}; |
| Catalytic activity: | |
Reaction=(S)-2,3,4,5-tetrahydrodipicolinate + H2O + NADP(+) = (2S,4S)- 4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H(+) + NADPH; Xref=Rhea:RHEA:35331, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16845, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:67139; EC=1.17.1.8; Evidence={ECO:0000255|HAMAP- Rule:MF_00102}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.2 uM for NADH {ECO:0000269|PubMed:12962488}; KM=11.8 uM for NADPH {ECO:0000269|PubMed:12962488}; |
| Pathway: | |
Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. {ECO:0000255|HAMAP-Rule:MF_00102}. |
| Subunit: | |
Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00102, ECO:0000269|PubMed:12962488, ECO:0000269|PubMed:16511139, ECO:0000269|PubMed:20057050}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00102}. |
| Similarity: | |
Belongs to the DapB family. {ECO:0000255|HAMAP- Rule:MF_00102}. |
Annotations taken from UniProtKB at the EBI.