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PDBsum entry 5tss
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Contents |
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* Residue conservation analysis
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References listed in PDB file
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Key reference
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Title
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Refined structures of three crystal forms of toxic shock syndrome toxin-1 and of a tetramutant with reduced activity.
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Authors
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G.S.Prasad,
R.Radhakrishnan,
D.T.Mitchell,
C.A.Earhart,
M.M.Dinges,
W.J.Cook,
P.M.Schlievert,
D.H.Ohlendorf.
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Ref.
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Protein Sci, 1997,
6,
1220-1227.
[DOI no: ]
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PubMed id
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Abstract
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The structure of toxic shock syndrome toxin-1 (TSST-1), the causative agent in
toxic shock syndrome, has been determined in three crystal forms. The three
structural models have been refined to R-factors of 0.154, 0.150, and 0.198 at
resolutions of 2.05 A, 2.90 A, and 2.75 A, respectively. One crystal form of
TSST-1 contains a zinc ion bound between two symmetry-related molecules.
Although not required for biological activity, zinc dramatically potentiates the
mitogenicity of TSST-1 at very low concentrations. In addition, the structure of
the tetramutant TSST-1H [T69I, Y80W, E132K, I140T], which is nonmitogenic and
does not amplify endotoxin shock, has been determined and refined in a fourth
crystal form (R-factor = 0.173 to 1.9 A resolution).
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Figure 1.
Fig. 1. Front view of TSST-I Numbers refer to /3 strands: letters refer to
helices. Key structural features are shown extracted from the rest of the
molecule.
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Figure 2.
Fig. 2. 2F, - F, electrondensitymapscontouredat I n. (A) RegionaroundPhe 131 in C2221 crystal form. Thesidechainbeyond
Cg was mittedfromthepositionalrefinementandphasecalculation. (B) Regionaround Zn 206 in P4,2)2 crystalform.Symmetry-
relatedmolecule is gray.
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The above figures are
reprinted
from an Open Access publication published by the Protein Society:
Protein Sci
(1997,
6,
1220-1227)
copyright 1997.
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Secondary reference #1
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Title
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Structure of toxic shock syndrome toxin 1.
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Authors
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G.S.Prasad,
C.A.Earhart,
D.L.Murray,
R.P.Novick,
P.M.Schlievert,
D.H.Ohlendorf.
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Ref.
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Biochemistry, 1993,
32,
13761-13766.
[DOI no: ]
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PubMed id
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