PDBsum entry 5tae

Hydrolase

PDB id: 5tae

Contents

Protein chain

316 a.a.

Ligands

VAL-LYS

PO4

Metals

_CA ×4

_ZN

Waters ×99

PDB id:

5tae

Name:

Hydrolase

Title:

Conformational sampling differences across the arrhenius plot biphasic break point at ambient temperature in the enzyme thermolysin

Structure:

Thermolysin. Chain: a. Fragment: unp residues 233-548. Synonym: thermostable neutral proteinase. Engineered: yes

Source:

Bacillus thermoproteolyticus. Organism_taxid: 1427. Gene: npr. Expressed in: bacillus subtilis. Expression_system_taxid: 1423

Resolution:

2.30Å R-factor: 0.154 R-free: 0.220

Authors:

M.Dong,B.J.Bahnson

Key ref:

M.Dong and b.j.bahnson Conformational sampling differences across the arrhen plot biphasic break point at ambient temperature in t enzyme thermolysin. To be published, .

Date:

09-Sep-16 Release date: 30-Aug-17

Protein chain

P00800  (THER_BACTH) -  Thermolysin from Bacillus thermoproteolyticus

Seq: 548 a.a.

Struc: 316 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.24.27 - thermolysin.
• Reaction: Preferential cleavage: Xaa-|-Leu > Xaa-|-Phe.
• Cofactor: Ca(2+); Zn(2+)