PDBsum entry 5szx

Transcription regulator/DNA

PDB id: 5szx

Contents

Protein chains

62 a.a.

DNA/RNA

Ligands

PO4

Waters ×14

PDB id:

5szx

Name:

Transcription regulator/DNA

Title:

Epstein-barr virus zta DNA binding domain homodimer in complex with methylated DNA

Structure:

DNA (5'-d( Tp Cp Tp Tp Cp Ap Tp (5Cm) p Gp Cp Tp Cp Ap Gp Tp Gp Cp T)-3'). Chain: c. Engineered: yes. DNA (5'-d( Ap Ap Gp Cp Ap Cp Tp Gp Ap Gp (5Cm) p Gp Ap Tp Gp Ap Ap G)-3'). Chain: d. Engineered: yes. Zta transcription factor.

Source:

Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Epstein-barr virus. Hhv-4, human herpesvirus 4. Organism_taxid: 10376. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.25Å R-factor: 0.261 R-free: 0.284

Authors:

S.Hong,J.R.Horton,X.Cheng

Key ref:

S.Hong et al. (2017). Methyl-dependent and spatial-specific DNA recognition by the orthologous transcription factors human AP-1 and Epstein-Barr virus Zta. Nucleic Acids Res, 45, 2503-2515. PubMed id: 28158710

Date:

15-Aug-16 Release date: 01-Mar-17

Protein chains

P03206  (BZLF1_EBVB9) -  Lytic switch protein BZLF1 from Epstein-Barr virus (strain B95-8)

Seq: 245 a.a.

Struc: 62 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

T-C-T-T-C-A-T-5CM-G-C-T-C-A-G-T-G-C-T 18 bases

A-A-G-C-A-C-T-G-A-G-5CM-G-A-T-G-A-A-G 18 bases

Nucleic Acids Res 45:2503-2515 (2017)

PubMed id: 28158710

Methyl-dependent and spatial-specific DNA recognition by the orthologous transcription factors human AP-1 and Epstein-Barr virus Zta.

S.Hong, D.Wang, J.R.Horton, X.Zhang, S.H.Speck, R.M.Blumenthal, X.Cheng.

ABSTRACT

No abstract given.