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UniProt functional annotation for P77933 | ||
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| UniProt code: P77933. |
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| Organism: | |
Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)). |
| Taxonomy: | |
Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae; Thermococcus. |
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| Function: | |
Intein encoded endonucleases are thought to mediate intein mobility by site-specific recombination initiated by endonuclease cleavage at the 'homing site' in gene that lack the intein. PI-PkoI recognizes 5'-GATTTAGATCCCTGTACC-3' and PI-PkoII recognizes 5'- CAGCTACTACGGTTAC-3'. |
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| Catalytic activity: | |
Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:11130, Rhea:RHEA-COMP:11131, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:83828; EC=2.7.7.7; |
| Biophysicochemical properties: | |
Temperature dependence: Thermostable.; |
| Ptm: | |
This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. {ECO:0000305}. |
| Biotechnology: | |
Pyrococcus kodakaraensis enzyme is the fastest thermostable DNA polymerase known today (130 nucleotides/s) and is used in high-performance PCR. |
| Similarity: | |
Belongs to the DNA polymerase type-B family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.