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PDBsum entry 5omf
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DNA binding protein
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PDB id
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5omf
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References listed in PDB file
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Key reference
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Title
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Crystal structures of ternary complexes of archaeal b-Family DNA polymerases.
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Authors
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H.M.Kropp,
K.Betz,
J.Wirth,
K.Diederichs,
A.Marx.
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Ref.
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PLoS One, 2017,
12,
e0188005.
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PubMed id
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Abstract
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Archaeal B-family polymerases drive biotechnology by accepting a wide substrate
range of chemically modified nucleotides. By now no structural data for archaeal
B-family DNA polymerases in a closed, ternary complex are available, which would
be the basis for developing next generation nucleotides. We present the ternary
crystal structures of KOD and 9°N DNA polymerases complexed with DNA and the
incoming dATP. The structures reveal a third metal ion in the active site, which
was so far only observed for the eukaryotic B-family DNA polymerase δ and no
other B-family DNA polymerase. The structures reveal a wide inner channel and
numerous interactions with the template strand that provide space for
modifications within the enzyme and may account for the high processivity,
respectively. The crystal structures provide insights into the superiority over
other DNA polymerases concerning the acceptance of modified nucleotides.
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