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PDBsum entry 5obc
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References listed in PDB file
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Key reference
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Title
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Ru-Based co releasing molecules with azole ligands: interaction with proteins and the co release mechanism disclosed by X-Ray crystallography.
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Authors
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N.Pontillo,
G.Ferraro,
L.Messori,
G.Tamasi,
A.Merlino.
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Ref.
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Dalton Trans, 2017,
46,
9621-9629.
[DOI no: ]
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PubMed id
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Abstract
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fac-[RuII(CO)3Cl2(N3-Imidazole)]
(RuIIIM),
fac-[RuII(CO)3Cl2(N3-methyl-imidazole)]
(RuIIMIM) and
fac-[RuII(CO)3Cl2(N3-methyl-benzimidazole)]
(RuIIMBI) are three ruthenium based CO releasing molecules (Ru-CORMs)
that are cytotoxic towards ovarian and colon carcinoma cell lines. Detailed
structural information on the adducts formed upon reaction of RuIIIM
and RuIIMIM with hen egg white lysozyme and of the three Ru-CORMs
with bovine pancreatic ribonuclease is provided here by X-ray crystallography.
Comparative analysis of seven crystal structures of these adducts allows one to
delineate some general trends in the reactivity of these Ru-CORMs with proteins.
Indeed, in all cases Ru-CORMs bind these model systems upon detachment of the
azole ligand and concomitant coordination to a protein His or Asp residue.
Apparently the three Ru-CORMs progressively dissociate losing azoles, chlorides,
and one or two CO molecules. Data were compared with those reported in the
literature for adducts of the same proteins with other Ru-CORMs and with
in-solution data previously obtained on the same systems. These results are
potentially useful for a better understanding of the chemistry, potential
toxicity and mechanism of actions of these interesting Ru-CORMs and are helpful
in defining the molecular mechanisms of CO release.
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