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PDBsum entry 5o1x
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Transcription
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PDB id
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5o1x
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References listed in PDB file
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Key reference
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Title
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The structure of transcription termination factor nrd1 reveals an original mode for guaa recognition.
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Authors
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E.Franco-Echevarría,
N.González-Polo,
S.Zorrilla,
S.Martínez-Lumbreras,
C.M.Santiveri,
R.Campos-Olivas,
M.Sánchez,
O.Calvo,
B.González,
J.M.Pérez-Cañadillas.
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Ref.
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Nucleic Acids Res, 2017,
45,
10293-10305.
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PubMed id
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Abstract
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Transcription termination of non-coding RNAs is regulated in yeast by a complex
of three RNA binding proteins: Nrd1, Nab3 and Sen1. Nrd1 is central in this
process by interacting with Rbp1 of RNA polymerase II, Trf4 of TRAMP and GUAA/G
terminator sequences. We lack structural data for the last of these binding
events. We determined the structures of Nrd1 RNA binding domain and its
complexes with three GUAA-containing RNAs, characterized RNA binding energetics
and tested rationally designed mutants in vivo. The Nrd1 structure shows an RRM
domain fused with a second α/β domain that we name split domain (SD), because
it is formed by two non-consecutive segments at each side of the RRM. The GUAA
interacts with both domains and with a pocket of water molecules, trapped
between the two stacking adenines and the SD. Comprehensive binding studies
demonstrate for the first time that Nrd1 has a slight preference for GUAA over
GUAG and genetic and functional studies suggest that Nrd1 RNA binding domain
might play further roles in non-coding RNAs transcription termination.
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