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PDBsum entry 5nxu
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Cell adhesion
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PDB id
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5nxu
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References listed in PDB file
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Key reference
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Title
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Porin self-Association enables cell-To-Cell contact inprovidencia stuartiifloating communities.
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Authors
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M.El-Khatib,
C.Nasrallah,
J.Lopes,
Q.T.Tran,
G.Tetreau,
H.Basbous,
D.Fenel,
B.Gallet,
M.Lethier,
J.M.Bolla,
J.M.Pagès,
M.Vivaudou,
M.Weik,
M.Winterhalter,
J.P.Colletier.
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Ref.
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Proc Natl Acad Sci U S A, 2018,
115,
E2220.
[DOI no: ]
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PubMed id
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Abstract
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The gram-negative pathogenProvidencia stuartiiforms floating communities
within which adjacent cells are in apparent contact, before depositing as
canonical surface-attached biofilms. Because porins are the most abundant
proteins in the outer membrane of gram-negative bacteria, we hypothesized that
they could be involved in cell-to-cell contact and undertook a
structure-function relationship study on the two porins ofP. stuartii,
Omp-Pst1 and Omp-Pst2. Our crystal structures reveal that these porins can
self-associate through their extracellular loops, forming dimers of trimers
(DOTs) that could enable cell-to-cell contact within floating communities.
Support for this hypothesis was obtained by studying the porin-dependent
aggregation of liposomes and model cells. The observation that facing channels
are open in the two porin structures suggests that DOTs could not only promote
cell-to-cell contact but also contribute to intercellular communication.
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