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PDBsum entry 5nss
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Transcription
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PDB id
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5nss
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Contents |
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238 a.a.
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1340 a.a.
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1352 a.a.
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74 a.a.
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(+ 0 more)
248 a.a.
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388 a.a.
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References listed in PDB file
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Key reference
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Title
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Structures of RNA polymerase closed and intermediate complexes reveal mechanisms of DNA opening and transcription initiation.
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Authors
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R.Glyde,
F.Ye,
V.C.Darbari,
N.Zhang,
M.Buck,
X.Zhang.
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Ref.
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Mol Cell, 2017,
67,
106.
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PubMed id
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Abstract
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Gene transcription is carried out by RNA polymerases (RNAPs). For transcription
to occur, the closed promoter complex (RPc), where DNA is double stranded, must
isomerize into an open promoter complex (RPo), where the DNA is melted out into
a transcription bubble and the single-stranded template DNA is delivered to the
RNAP active site. Using a bacterial RNAP containing the alternative
σ54factor and cryoelectron microscopy, we determined structures of
RPc and the activator-bound intermediate complex en route to RPo at 3.8 and
5.8 Å. Our structures show how RNAP-σ54interacts with promoter DNA
to initiate the DNA distortions required for transcription bubble formation, and
how the activator interacts with RPc, leading to significant conformational
changes in RNAP and σ54that promote RPo formation. We propose that
DNA melting is an active process initiated in RPc and that the RNAP
conformations of intermediates are significantly different from that of RPc and
RPo.
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