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UniProt functional annotation for M1GRN3 | ||
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| UniProt code: M1GRN3. |
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| Organism: | |
Lactobacillus buchneri. |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Lentilactobacillus. |
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| Function: | |
Catalyzes the epimerization of L-isoleucine to D-allo- isoleucine and D-allo-isoleucine to L-isoleucine. Can also catalyze the racemization of many nonpolar amino acids, including leucine and valine. Does not have GABA aminotransferase activity. {ECO:0000269|PubMed:24039265}. |
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| Catalytic activity: | |
Reaction=L-isoleucine = D-allo-isoleucine; Xref=Rhea:RHEA:45560, ChEBI:CHEBI:58045, ChEBI:CHEBI:85306; EC=5.1.1.21; Evidence={ECO:0000269|PubMed:24039265}; |
| Cofactor: | |
Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326; Evidence={ECO:0000269|PubMed:24039265}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=5.0 mM for L-isoleucine {ECO:0000269|PubMed:24039265}; KM=13.2 mM for D-allo-isoleucine {ECO:0000269|PubMed:24039265}; Vmax=153 umol/min/mg enzyme for the forward reaction {ECO:0000269|PubMed:24039265}; Vmax=286 umol/min/mg enzyme for the reverse reaction {ECO:0000269|PubMed:24039265}; Note=kcat is 502 sec(-1) with L-isoleucine as substrate. kcat is 939 sec(-1) with D-allo-isoleucine as substrate. {ECO:0000269|PubMed:24039265}; pH dependence: Optimum pH is 5.0 for the forward reaction, and 6.0 for the reverse reaction. {ECO:0000269|PubMed:24039265}; Temperature dependence: Optimum temperature is 50 degrees Celsius for the forward reaction, and 45 degrees Celsius for the reverse reaction. {ECO:0000269|PubMed:24039265}; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:24039265}. |
| Similarity: | |
Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.