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UniProt functional annotation for G2QG48 | ||
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| UniProt code: G2QG48. |
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| Organism: | |
Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) (Sporotrichum thermophile). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Sordariales; Chaetomiaceae; Thermothelomyces. |
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| Function: | |
Catalyzes the selective oxidation of C1 hydroxyl moieties on mono-, oligo- and polysaccharides with concomitant reduction of molecular oxygen to hydrogen peroxide. This results in the formation of the corresponding lactones, which typically undergo spontaneous hydrolysis. Xylooligosaccharide oxidase is able to oxidize a variety of substrates including D-xylose, D-cellobiose, lactose and arabinose. The enzyme acts primarily on xylooligosaccharides, indicating that it prefers pentose-based oligosaccharides over hexose-based oligosaccharides. {ECO:0000269|PubMed:27629413}. |
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| Catalytic activity: | |
Reaction=D-xylobiose + O2 = D-xylobiono-1,5-lactone + H2O2; Xref=Rhea:RHEA:59632, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:28309, ChEBI:CHEBI:143156; Evidence={ECO:0000269|PubMed:27629413}; |
| Catalytic activity: | |
Reaction=D-xylotriose + O2 = D-xylotriono-1,5-lactone + H2O2; Xref=Rhea:RHEA:59636, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:62783, ChEBI:CHEBI:143164; Evidence={ECO:0000269|PubMed:27629413}; |
| Catalytic activity: | |
Reaction=D-xylotetraose + O2 = D-xylotetraono-1,5-lactone + H2O2; Xref=Rhea:RHEA:59640, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:62972, ChEBI:CHEBI:143165; Evidence={ECO:0000269|PubMed:27629413}; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:27629413}; Note=Binds 1 FAD per subunit in a bicovalent manner. {ECO:0000269|PubMed:27629413}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.13 mM for oxygen {ECO:0000269|PubMed:27629413}; KM=342 mM for cellobiose {ECO:0000269|PubMed:27629413}; KM=532 mM for lactose {ECO:0000269|PubMed:27629413}; KM=359 mM for xylose {ECO:0000269|PubMed:27629413}; KM=1.15 mM for xylobiose {ECO:0000269|PubMed:27629413}; KM=0.69 mM for xylotriose {ECO:0000269|PubMed:27629413}; KM=0.43 mM for xylotetraose {ECO:0000269|PubMed:27629413}; pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:27629413}; Temperature dependence: Optimum temperature is 30 degrees Celsius. Stable up to 60 degrees Celsius. {ECO:0000269|PubMed:27629413}; |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:27629413}. |
| Ptm: | |
The FAD cofactor is bound via a bicovalent 6-S-cysteinyl, 8alpha- N1-histidyl FAD linkage. {ECO:0000269|PubMed:27629413}. |
| Similarity: | |
Belongs to the oxygen-dependent FAD-linked oxidoreductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.