UniProt functional annotation for P74334



	UniProt functional annotation for P74334

UniProt code: P74334.

Organism: Synechocystis sp. (strain PCC 6803 / Kazusa).

Taxonomy: Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis; unclassified Synechocystis.

Function: Cleaves a number of carotenals and carotenols in the all- trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta-carotene or lycopene.

Catalytic activity: Reaction=all-trans-8'-apo-beta-carotenal + O2 = (2E,4E,6E)-2,6- dimethylocta-2,4,6-trienedial + all-trans-retinal; Xref=Rhea:RHEA:26385, ChEBI:CHEBI:15379, ChEBI:CHEBI:17898, ChEBI:CHEBI:53154, ChEBI:CHEBI:53155; EC=1.13.11.75; Evidence={ECO:0000269|PubMed:15686550};

Cofactor: Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:15821095}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000269|PubMed:15821095};

Biophysicochemical properties: Kinetic parameters: KM=2.5 uM for 8'-apo-beta-carotenal {ECO:0000269|PubMed:15686550}; KM=43 uM for 8'-apo-beta-carotenol {ECO:0000269|PubMed:15686550}; KM=1.4 uM for (3R)-3-OH-8'-apo-beta-carotenal {ECO:0000269|PubMed:15686550}; KM=31 uM for (3R)-3-OH-8'-apo-beta-carotenol {ECO:0000269|PubMed:15686550}; KM=2.6 uM for (3R)-3-OH-12'-apo-beta-carotenal {ECO:0000269|PubMed:15686550}; Vmax=40 pmol/min/mg enzyme with 8'-apo-beta-carotenal as substrate {ECO:0000269|PubMed:15686550}; Vmax=650 pmol/min/mg enzyme with 8'-apo-beta-carotenol as substrate {ECO:0000269|PubMed:15686550}; Vmax=71 pmol/min/mg enzyme with (3R)-3-OH-8'-apo-beta-carotenal as substrate {ECO:0000269|PubMed:15686550}; Vmax=705 pmol/min/mg enzyme with (3R)-3-OH-8'-apo-beta-carotenol as substrate {ECO:0000269|PubMed:15686550}; Vmax=11.5 pmol/min/mg enzyme with (3R)-3-OH-12'-apo-beta-carotenal as substrate {ECO:0000269|PubMed:15686550};

Induction: By high light. {ECO:0000269|PubMed:15686550}.

Similarity: Belongs to the carotenoid oxygenase family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Synechocystis sp. (strain PCC 6803 / Kazusa).
Taxonomy:		Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis; unclassified Synechocystis.



Function:		Cleaves a number of carotenals and carotenols in the all- trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta-carotene or lycopene.


Catalytic activity:		Reaction=all-trans-8'-apo-beta-carotenal + O2 = (2E,4E,6E)-2,6- dimethylocta-2,4,6-trienedial + all-trans-retinal; Xref=Rhea:RHEA:26385, ChEBI:CHEBI:15379, ChEBI:CHEBI:17898, ChEBI:CHEBI:53154, ChEBI:CHEBI:53155; EC=1.13.11.75; Evidence={ECO:0000269\|PubMed:15686550};
Cofactor:		Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269\|PubMed:15821095}; Note=Binds 1 Fe(2+) ion per subunit. {ECO:0000269\|PubMed:15821095};
Biophysicochemical properties:		Kinetic parameters: KM=2.5 uM for 8'-apo-beta-carotenal {ECO:0000269\|PubMed:15686550}; KM=43 uM for 8'-apo-beta-carotenol {ECO:0000269\|PubMed:15686550}; KM=1.4 uM for (3R)-3-OH-8'-apo-beta-carotenal {ECO:0000269\|PubMed:15686550}; KM=31 uM for (3R)-3-OH-8'-apo-beta-carotenol {ECO:0000269\|PubMed:15686550}; KM=2.6 uM for (3R)-3-OH-12'-apo-beta-carotenal {ECO:0000269\|PubMed:15686550}; Vmax=40 pmol/min/mg enzyme with 8'-apo-beta-carotenal as substrate {ECO:0000269\|PubMed:15686550}; Vmax=650 pmol/min/mg enzyme with 8'-apo-beta-carotenol as substrate {ECO:0000269\|PubMed:15686550}; Vmax=71 pmol/min/mg enzyme with (3R)-3-OH-8'-apo-beta-carotenal as substrate {ECO:0000269\|PubMed:15686550}; Vmax=705 pmol/min/mg enzyme with (3R)-3-OH-8'-apo-beta-carotenol as substrate {ECO:0000269\|PubMed:15686550}; Vmax=11.5 pmol/min/mg enzyme with (3R)-3-OH-12'-apo-beta-carotenal as substrate {ECO:0000269\|PubMed:15686550};
Induction:		By high light. {ECO:0000269\|PubMed:15686550}.
Similarity:		Belongs to the carotenoid oxygenase family. {ECO:0000305}.