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PDBsum entry 5kd1
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Oxygen transport
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PDB id
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5kd1
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References listed in PDB file
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Key reference
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Title
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Nitrosoamphetamine binding to myoglobin and hemoglobin: crystal structure of the h64a myoglobin-Nitrosoamphetamine adduct.
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Authors
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B.Wang,
S.M.Powell,
Y.Guan,
N.Xu,
L.M.Thomas,
G.B.Richter-Addo.
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Ref.
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Nitric Oxide, 2017,
67,
26-29.
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PubMed id
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Abstract
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N-hydroxyamphetamine (AmphNHOH) is an oxidative metabolite of amphetamine and
methamphetamine. It is known to form inhibitory complexes upon binding to heme
proteins. However, its interactions with myoglobin (Mb) and hemoglobin (Hb) have
not been reported. We demonstrate that the reactions of AmphNHOH with ferric Mb
and Hb generate the respective heme-nitrosoamphetamine derivatives characterized
by UV-vis spectroscopy. We have determined the X-ray crystal structure of the
H64A Mb-nitrosoamphetamine complex to 1.73 Å resolution. The structure reveals
the N-binding of the nitroso-d-amphetamine isomer, with no significant H-bonding
interactions between the ligand and the distal pocket amino acid residues.
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