PDBsum entry 5k5a

Hydrolase

PDB id: 5k5a

Contents

Protein chain

268 a.a.

PDB id:

5k5a

Name:

Hydrolase

Title:

Structure of the pnob8-like parb n-domain

Structure:

Parb domain protein nuclease. Chain: b. Fragment: unp residues 2-287. Engineered: yes

Source:

Sulfolobus solfataricus. Organism_taxid: 555311. Strain: 98/2. Gene: ssol_1539. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.83Å R-factor: 0.236 R-free: 0.276

Authors:

M.Schumacher

Key ref:

M.A.Schumacher et al. (2015). Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages. Science, 349, 1120-1124. PubMed id: 26339031 DOI: 10.1126/science.aaa9046

Date:

23-May-16 Release date: 15-Jun-16

Supersedes:

4rsf

Protein chain

D0KSP7  (D0KSP7_SACS9) -  ParB domain protein nuclease from Saccharolobus solfataricus (strain 98/2)

Seq: 350 a.a.

Struc: 268 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DOI no: 10.1126/science.aaa9046

Science 349:1120-1124 (2015)

PubMed id: 26339031

Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.

M.A.Schumacher, N.K.Tonthat, J.Lee, F.A.Rodriguez-Castañeda, N.B.Chinnam, A.K.Kalliomaa-Sanford, I.W.Ng, M.T.Barge, P.L.Shaw, D.Barillà.

ABSTRACT

Although recent studies have provided a wealth of information about archaeal biology, nothing is known about the molecular basis of DNA segregation in these organisms. Here, we unveil the machinery and assembly mechanism of the archaeal Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a spreading mechanism to create a DNA superhelix onto which ParB assembles. This supercomplex links to the ParA motor, which contains a bacteria-like Walker motif. The C domain of ParB harbors structural similarity to CenpA, which dictates eukaryotic segregation. Thus, this archaeal system combines bacteria-like and eukarya-like components, which suggests the possible conservation of DNA segregation principles across the three domains of life.