 |
PDBsum entry 5k5a
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Structures of archaeal DNA segregation machinery reveal bacterial and eukaryotic linkages.
|
|
|
Authors
|
|
M.A.Schumacher,
N.K.Tonthat,
J.Lee,
F.A.Rodriguez-Castañeda,
N.B.Chinnam,
A.K.Kalliomaa-Sanford,
I.W.Ng,
M.T.Barge,
P.L.Shaw,
D.Barillà.
|
|
|
Ref.
|
|
Science, 2015,
349,
1120-1124.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Although recent studies have provided a wealth of information about archaeal
biology, nothing is known about the molecular basis of DNA segregation in these
organisms. Here, we unveil the machinery and assembly mechanism of the archaeal
Sulfolobus pNOB8 partition system. This system uses three proteins: ParA; an
atypical ParB adaptor; and a centromere-binding component, AspA. AspA utilizes a
spreading mechanism to create a DNA superhelix onto which ParB assembles. This
supercomplex links to the ParA motor, which contains a bacteria-like Walker
motif. The C domain of ParB harbors structural similarity to CenpA, which
dictates eukaryotic segregation. Thus, this archaeal system combines
bacteria-like and eukarya-like components, which suggests the possible
conservation of DNA segregation principles across the three domains of life.
|
|
|
|
|
|
|
