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UniProt functional annotation for P11454 | ||
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| UniProt code: P11454. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Activates the carboxylate group of L-serine via ATP-dependent PPi exchange reactions to the aminoacyladenylate, preparing that molecule for the final stages of enterobactin synthesis. Holo-EntF acts as the catalyst for the formation of the three amide and three ester bonds present in the cyclic (2,3-dihydroxybenzoyl)serine trimer enterobactin, using seryladenylate and acyl-holo-EntB (acylated with 2,3-dihydroxybenzoate by EntE). |
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| Catalytic activity: | |
Reaction=ATP + L-serine = diphosphate + L-seryl-5'-AMP; Xref=Rhea:RHEA:28570, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:33384, ChEBI:CHEBI:61231; |
| Cofactor: | |
Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942; Note=Binds 1 phosphopantetheine covalently.; |
| Pathway: | |
Siderophore biosynthesis; enterobactin biosynthesis. |
| Subunit: | |
EntB, EntD, EntE, and EntF form a multienzyme complex called enterobactin synthase. EntF acts as a catalytic monomer. |
| Induction: | |
Transcriptionally regulated by iron and the fur protein. |
| Ptm: | |
4'-phosphopantetheine is transferred from CoA to a specific serine of apo-EntF by EntD. Holo-EntF so formed is then acylated with seryl- AMP. |
| Similarity: | |
Belongs to the ATP-dependent AMP-binding enzyme family. EntF subfamily. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAB40785.1; Type=Erroneous initiation; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.