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PDBsum entry 5iv8
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Transport protein
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PDB id
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5iv8
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References listed in PDB file
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Key reference
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Title
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Structural and functional characterization of the lps transporter lptde from gram-Negative pathogens.
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Authors
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I.Botos,
N.Majdalani,
S.J.Mayclin,
J.G.Mccarthy,
K.Lundquist,
D.Wojtowicz,
T.J.Barnard,
J.C.Gumbart,
S.K.Buchanan.
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Ref.
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Structure, 2016,
24,
965-976.
[DOI no: ]
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PubMed id
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Abstract
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Incorporation of lipopolysaccharide (LPS) into the outer membrane of
Gram-negative bacteria is essential for viability, and is accomplished by a
two-protein complex called LptDE. We solved crystal structures of the core LptDE
complexes from Yersinia pestis, Klebsiella pneumoniae, Pseudomonas aeruginosa,
and a full-length structure of the K. pneumoniae LptDE complex. Our structures
adopt the same plug and 26-strand β-barrel architecture found recently for the
Shigella flexneri and Salmonella typhimurium LptDE structures, illustrating a
conserved fold across the family. A comparison of the only two full-length
structures, SfLptDE and our KpLptDE, reveals a 21° rotation of the LptD
N-terminal domain that may impart flexibility on the trans-envelope LptCAD
scaffold. Utilizing mutagenesis coupled to an in vivo functional assay and
molecular dynamics simulations, we demonstrate the critical role of Pro231 and
Pro246 in the function of the LptD lateral gate that allows partitioning of LPS
into the outer membrane.
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