 |
PDBsum entry 5hqd
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Acoustic injectors for drop-On-Demand serial femtosecond crystallography.
|
|
|
Authors
|
|
C.G.Roessler,
R.Agarwal,
M.Allaire,
R.Alonso-Mori,
B.Andi,
J.F.Bachega,
M.Bommer,
A.S.Brewster,
M.C.Browne,
R.Chatterjee,
E.Cho,
A.E.Cohen,
M.Cowan,
S.Datwani,
V.L.Davidson,
J.Defever,
B.Eaton,
R.Ellson,
Y.Feng,
L.P.Ghislain,
J.M.Glownia,
G.Han,
J.Hattne,
J.Hellmich,
A.Héroux,
M.Ibrahim,
J.Kern,
A.Kuczewski,
H.T.Lemke,
P.Liu,
L.Majlof,
W.M.Mcclintock,
S.Myers,
S.Nelsen,
J.Olechno,
A.M.Orville,
N.K.Sauter,
A.S.Soares,
S.M.Soltis,
H.Song,
R.G.Stearns,
R.Tran,
Y.Tsai,
M.Uervirojnangkoorn,
C.M.Wilmot,
V.Yachandra,
J.Yano,
E.T.Yukl,
D.Zhu,
A.Zouni.
|
|
|
Ref.
|
|
Structure, 2016,
24,
631-640.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
Abstract
|
|
|
X-ray free-electron lasers (XFELs) provide very intense X-ray pulses suitable
for macromolecular crystallography. Each X-ray pulse typically lasts for tens of
femtoseconds and the interval between pulses is many orders of magnitude longer.
Here we describe two novel acoustic injection systems that use focused sound
waves to eject picoliter to nanoliter crystal-containing droplets out of
microplates and into the X-ray pulse from which diffraction data are collected.
The on-demand droplet delivery is synchronized to the XFEL pulse scheme,
resulting in X-ray pulses intersecting up to 88% of the droplets. We tested
several types of samples in a range of crystallization conditions, wherein the
overall crystal hit ratio (e.g., fraction of images with observable diffraction
patterns) is a function of the microcrystal slurry concentration. We report
crystal structures from lysozyme, thermolysin, and stachydrine demethylase
(Stc2). Additional samples were screened to demonstrate that these methods can
be applied to rare samples.
|
|
|
|
|
|
|
