PDBsum entry 5g4i

Transferase

PDB id: 5g4i

Contents

Protein chains

423 a.a.

Ligands

PLP ×2

PO4 ×5

ACT ×3

B3P

Waters ×665

PDB id:

5g4i

Name:

Transferase

Title:

Plp-dependent phospholyase a1rdf1 from arthrobacter aurescens tc1

Structure:

Phospholyase. Chain: a, b. Engineered: yes

Source:

Arthrobacter aurescens. Organism_taxid: 290340. Strain: tc1. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.50Å R-factor: 0.154 R-free: 0.175

Authors:

A.Cuetos,A.N.Tuan,J.Mangas Sanchez,G.Grogan

Key ref:

A.Cuetos et al. (2016). Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue. Chembiochem, 17, 2308-2311. PubMed id: 27709756 DOI: 10.1002/cbic.201600482

Date:

13-May-16 Release date: 19-Oct-16

Protein chains

A1RDF1  (A1RDF1_PAEAT) -  Putative Aminotransferase class III protein from Paenarthrobacter aurescens (strain TC1)

Seq: 446 a.a.

Struc: 423 a.a.

Enzyme reactions

• Enzyme class: E.C.4.2.3.2 - ethanolamine-phosphate phospho-lyase.
• Reaction: phosphoethanolamine + H2O = acetaldehyde + NH4⁺ + phosphate

phosphoethanolamine + H2O = acetaldehyde (Bound ligand (Het Group name = ACT) matches with 75.00% similarity) + NH4(+) + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly)

• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) matches with 93.75% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1002/cbic.201600482

Chembiochem 17:2308-2311 (2016)

PubMed id: 27709756

Structural Basis for Phospholyase Activity of a Class III Transaminase Homologue.

A.Cuetos, F.Steffen-Munsberg, J.Mangas Sanchez, A.Frese, U.T.Bornscheuer, M.Höhne, G.Grogan.

ABSTRACT

No abstract given.