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UniProt functional annotation for P17109 | ||
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| UniProt code: P17109. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2- succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). {ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000269|PubMed:17760421}. |
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| Catalytic activity: | |
Reaction=2-oxoglutarate + H(+) + isochorismate = 5-enolpyruvoyl-6- hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2; Xref=Rhea:RHEA:25593, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:29780, ChEBI:CHEBI:58818; EC=2.2.1.9; Evidence={ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000269|PubMed:17760421}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01659}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000255|HAMAP-Rule:MF_01659}; |
| Cofactor: | |
Name=thiamine diphosphate; Xref=ChEBI:CHEBI:58937; Evidence={ECO:0000255|HAMAP-Rule:MF_01659}; Note=Binds 1 thiamine pyrophosphate per subunit. {ECO:0000255|HAMAP- Rule:MF_01659}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=53 nM for isochorismate (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; KM=1.5 uM for 2-oxoglutarate (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; KM=2.4 uM for thiamine diphosphate (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; KM=80 uM for magnesium ions (at 22.5 degrees Celsius and pH 7.8) {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; pH dependence: Optimum pH is 7-8. {ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:17760421}; |
| Pathway: | |
Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 2/7. {ECO:0000255|HAMAP-Rule:MF_01659}. |
| Pathway: | |
Quinol/quinone metabolism; menaquinone biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01659}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000269|PubMed:14621995, ECO:0000269|PubMed:16511076}. |
| Induction: | |
By isopropyl-beta-D-thiogalactoside (IPTG). {ECO:0000269|PubMed:1459959}. |
| Similarity: | |
Belongs to the TPP enzyme family. MenD subfamily. {ECO:0000255|HAMAP-Rule:MF_01659, ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA24153.1; Type=Frameshift; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.