PDBsum entry 5eig

Lyase

PDB id: 5eig

Contents

Protein chains

(+ 2 more) 384 a.a.

Ligands

SO4 ×5

CYS ×3

Waters ×666

PDB id:

5eig

Name:

Lyase

Title:

Engineered human cystathionine gamma lyase (e59t, e339v) to deplet cysteine

Structure:

Cystathionine gamma-lyase. Chain: a, c, d, e, f, g. Synonym: cysteine-protein sulfhydrase,gamma-cystathionase. Engineered: yes. Mutation: yes. Cystathionine gamma-lyase. Chain: b, h. Synonym: cysteine-protein sulfhydrase,gamma-cystathionase. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cth. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_taxid: 562

Resolution:

2.70Å R-factor: 0.193 R-free: 0.233

Authors:

W.Yan,J.Zhang

Key ref:

S.Cramer et al. Systemic depletion of serum l-Cyst(e)ine with an engi human enzyme mediates potent induction of ros and can ablation. To be published, .

Date:

29-Oct-15 Release date: 09-Nov-16

Protein chains

P32929  (CGL_HUMAN) -  Cystathionine gamma-lyase from Homo sapiens

Seq: 405 a.a.

Struc: 384 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: E.C.4.4.1.1 - cystathionine gamma-lyase.
• Reaction: L,L-cystathionine + H2O = 2-oxobutanoate + L-cysteine + NH4⁺

L,L-cystathionine + H2O = 2-oxobutanoate + L-cysteine (Bound ligand (Het Group name = CYS) corresponds exactly) + NH4(+)

• Cofactor: Pyridoxal 5'-phosphate
• Enzyme class 2: E.C.4.4.1.2 - homocysteine desulfhydrase.
• Reaction: L-homocysteine + H2O = 2-oxobutanoate + hydrogen sulfide + NH4⁺ + H⁺

L-homocysteine (Bound ligand (Het Group name = CYS) matches with 66.67% similarity) + H2O = 2-oxobutanoate + hydrogen sulfide + NH4(+) + H(+)

• Cofactor: Pyridoxal 5'-phosphate

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site