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PDBsum entry 5efw
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Signaling protein
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PDB id
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5efw
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References listed in PDB file
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Key reference
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Title
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Lovtrap: an optogenetic system for photoinduced protein dissociation.
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Authors
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H.Wang,
M.Vilela,
A.Winkler,
M.Tarnawski,
I.Schlichting,
H.Yumerefendi,
B.Kuhlman,
R.Liu,
G.Danuser,
K.M.Hahn.
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Ref.
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Nat Methods, 2016,
13,
755-758.
[DOI no: ]
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PubMed id
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Abstract
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LOVTRAP is an optogenetic approach for reversible light-induced protein
dissociation using protein A fragments that bind to the LOV domain only in the
dark, with tunable kinetics and a >150-fold change in the dissociation
constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely
modulated the proteins' access to the cell edge, demonstrating a naturally
occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and
PI3K proteins.
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