UniProt functional annotation for Q8L7C9



	UniProt functional annotation for Q8L7C9

UniProt code: Q8L7C9.

Organism: Arabidopsis thaliana (Mouse-ear cress).

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.

Function: Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development (PubMed:17220357). Regulator of the interplay between light and JA signaling by increasing JAR1/FIN219 efficiency (PubMed:28223489). Maybe involved in gravitropic signal transduction (Probable). {ECO:0000269|PubMed:17220357, ECO:0000269|PubMed:28223489, ECO:0000305|PubMed:23281391}.

Catalytic activity: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269|PubMed:17220357, ECO:0000269|PubMed:28223489};

Activity regulation: Activated by JAR1/FIN219. {ECO:0000269|PubMed:28223489}.

Biophysicochemical properties: Kinetic parameters: KM=0.467 mM for glutathione {ECO:0000269|PubMed:17220357}; KM=1.794 mM for 1-chloro-2,4- dinitrobenzene {ECO:0000269|PubMed:17220357};

Subunit: Homodimerization. Interacts with JAR1/FIN219 under continuous far red (cFR) light to stimulate JAR1/FIN219 activity and substrate selectivity. {ECO:0000269|PubMed:17220357, ECO:0000269|PubMed:28223489}.

Subcellular location: Nucleus {ECO:0000269|PubMed:17220357}. Cytoplasm, cytosol {ECO:0000269|PubMed:17220357}.

Tissue specificity: Mostly associated with vascular tissues, especially near hydathodes. {ECO:0000269|PubMed:17220357}.

Developmental stage: Light-dependent expression. Developmentally regulated. First observed in cotyledon vascular tissues of young seedlings. Appears at the shoot apex, in the upper part of hypocotyls and in roots in one week old seedlings. Later highly expressed in the basal portion of trichomes, veins, shoot apex, and hypocotyls. Becomes restricted to the margins of leaves and in roots. In drakness and blue light (B) grown plants, localized in cotyledons vascular tissues. In far-red (FR) and red (R) light conditions, mostly confined to regions of vascular tissues near the hydathode of cotyledons. In adult plants, expressed in vascular tissues of flower organs. {ECO:0000269|PubMed:17220357}.

Induction: Induced by a gravistimulation. {ECO:0000269|PubMed:23281391}.

Disruption phenotype: Hyposensitive hypocotyl phenotype under continuous far red (cFR) light and a delayed flowering phenotype under long-day conditions. {ECO:0000269|PubMed:17220357}.

Similarity: Belongs to the GST superfamily. Tau family. {ECO:0000305}.

Sequence caution: Sequence=AAF71798.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Arabidopsis thaliana (Mouse-ear cress).
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.



Function:		Exhibits glutathione-dependent thiol transferase activities. Can use glutathione (GSH) and 1-chloro-2,4-dinitrobenzene (CDNB) as substrates. Involved in the regulation of far-red light influence on development (PubMed:17220357). Regulator of the interplay between light and JA signaling by increasing JAR1/FIN219 efficiency (PubMed:28223489). Maybe involved in gravitropic signal transduction (Probable). {ECO:0000269\|PubMed:17220357, ECO:0000269\|PubMed:28223489, ECO:0000305\|PubMed:23281391}.


Catalytic activity:		Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; Evidence={ECO:0000269\|PubMed:17220357, ECO:0000269\|PubMed:28223489};
Activity regulation:		Activated by JAR1/FIN219. {ECO:0000269\|PubMed:28223489}.
Biophysicochemical properties:		Kinetic parameters: KM=0.467 mM for glutathione {ECO:0000269\|PubMed:17220357}; KM=1.794 mM for 1-chloro-2,4- dinitrobenzene {ECO:0000269\|PubMed:17220357};
Subunit:		Homodimerization. Interacts with JAR1/FIN219 under continuous far red (cFR) light to stimulate JAR1/FIN219 activity and substrate selectivity. {ECO:0000269\|PubMed:17220357, ECO:0000269\|PubMed:28223489}.
Subcellular location:		Nucleus {ECO:0000269\|PubMed:17220357}. Cytoplasm, cytosol {ECO:0000269\|PubMed:17220357}.
Tissue specificity:		Mostly associated with vascular tissues, especially near hydathodes. {ECO:0000269\|PubMed:17220357}.
Developmental stage:		Light-dependent expression. Developmentally regulated. First observed in cotyledon vascular tissues of young seedlings. Appears at the shoot apex, in the upper part of hypocotyls and in roots in one week old seedlings. Later highly expressed in the basal portion of trichomes, veins, shoot apex, and hypocotyls. Becomes restricted to the margins of leaves and in roots. In drakness and blue light (B) grown plants, localized in cotyledons vascular tissues. In far-red (FR) and red (R) light conditions, mostly confined to regions of vascular tissues near the hydathode of cotyledons. In adult plants, expressed in vascular tissues of flower organs. {ECO:0000269\|PubMed:17220357}.
Induction:		Induced by a gravistimulation. {ECO:0000269\|PubMed:23281391}.
Disruption phenotype:		Hyposensitive hypocotyl phenotype under continuous far red (cFR) light and a delayed flowering phenotype under long-day conditions. {ECO:0000269\|PubMed:17220357}.
Similarity:		Belongs to the GST superfamily. Tau family. {ECO:0000305}.
Sequence caution:		Sequence=AAF71798.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};