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PDBsum entry 5ec6
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Metal transport
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PDB id
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5ec6
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References listed in PDB file
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Key reference
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Title
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Structural analysis of haemoglobin binding by hpua from the neisseriaceae family.
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Authors
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C.T.Wong,
Y.Xu,
A.Gupta,
J.A.Garnett,
S.J.Matthews,
S.A.Hare.
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Ref.
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Nat Commun, 2015,
6,
10172.
[DOI no: ]
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PubMed id
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Abstract
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The Neisseriaceae family of bacteria causes a range of diseases including
meningitis, septicaemia, gonorrhoea and endocarditis, and extracts haem from
haemoglobin as an important iron source within the iron-limited environment of
its human host. Herein we report crystal structures of apo- and
haemoglobin-bound HpuA, an essential component of this haem import system. The
interface involves long loops on the bacterial receptor that present hydrophobic
side chains for packing against the surface of haemoglobin. Interestingly, our
structural and biochemical analyses of Kingella denitrificans and Neisseria
gonorrhoeae HpuA mutants, although validating the interactions observed in the
crystal structure, show how Neisseriaceae have the fascinating ability to
diversify functional sequences and yet retain the haemoglobin binding function.
Our results present the first description of HpuA's role in direct binding of
haemoglobin.
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