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PDBsum entry 5ebd
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Protein transport
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PDB id
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5ebd
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References listed in PDB file
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Key reference
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Title
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Crystallographic observation of the movement of the membrane-Distal domain of the t7ss core component eccb1 from mycobacterium tuberculosis.
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Authors
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X.Q.Xie,
X.L.Zhang,
C.Qi,
D.F.Li,
J.Fleming,
D.C.Wang,
L.J.Bi.
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Ref.
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Acta Crystallogr F Struct Biol Commun, 2016,
72,
139-144.
[DOI no: ]
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PubMed id
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Abstract
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The protein EccB1, a core component of the type VII secretion system (T7SS) of
Mycobacterium tuberculosis, has been identified as an ATPase and is essential
for the secretion of virulence factors by the ESX-1 system. In a previous study,
EccB1 structures were determined in two different conformations. Here, two new
conformations are identified and described. These four conformations present
snapshots of the swinging movement of the membrane-distal domain A2. The
movement of this domain involves conformational changes in two flexible loops
(loop A, residues 243-264, and loop B, residues 324-341) which are rich in
proline and glycine residues and connect domain A2 to domains C1 and B2. It is
proposed that the movement of this domain is related to the ATPase activity of
EccB1 and its homologues, as well as to the substrate transport of ESX secretion
systems.
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