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UniProt functional annotation for Q40577 | ||
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| UniProt code: Q40577. |
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| Organism: | |
Nicotiana tabacum (Common tobacco). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae; Nicotiana. |
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| Function: | |
Catalyzes the cyclization of trans,trans-farnesyl diphosphate (FPP) to the bicyclic intermediate 5-epi-aristolochene, initial step in the conversion of FPP to the sesquiterpenoid antifungal phytoalexin capsidiol (Ref.3, PubMed:16375847). Produces germacrene A as an enzyme- bound intermediate that is not released by the enzyme, but is further cyclized to produce the bicyclic 5-epi-aristolochene (Ref.3, PubMed:16375847). {ECO:0000269|PubMed:16375847, ECO:0000269|Ref.3}. |
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| Catalytic activity: | |
Reaction=(2E,6E)-farnesyl diphosphate = (+)-5-epi-aristolochene + diphosphate; Xref=Rhea:RHEA:28635, ChEBI:CHEBI:23925, ChEBI:CHEBI:33019, ChEBI:CHEBI:175763; EC=4.2.3.61; Evidence={ECO:0000269|PubMed:16375847, ECO:0000269|Ref.3}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28636; Evidence={ECO:0000269|PubMed:16375847, ECO:0000269|Ref.3}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:9295271}; Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000269|PubMed:9295271}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=2.3 uM for 2-trans,6-trans-farnesyl diphosphate {ECO:0000269|Ref.3}; |
| Pathway: | |
Secondary metabolite biosynthesis; terpenoid biosynthesis. {ECO:0000305}. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:9295271}. |
| Subcellular location: | |
Cytoplasm. |
| Induction: | |
By fungal elicitor. {ECO:0000269|PubMed:1438319}. |
| Domain: | |
The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for the catalytic activity, presumably through binding to Mg(2+). {ECO:0000305}. |
| Miscellaneous: | |
PubMed:1438319 indicates the presence of at least two genes coding for the same protein. |
| Similarity: | |
Belongs to the terpene synthase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.