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UniProt functional annotation for P0CT06 | ||
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| UniProt code: P0CT06. |
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| Organism: | |
Magnaporthe oryzae (strain 70-15 / ATCC MYA-4617 / FGSC 8958) (Rice blast fungus) (Pyricularia oryzae). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Magnaporthales; Pyriculariaceae; Pyricularia. |
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| Function: | |
Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2- methylisocitrate (By similarity). plays an important role in plant pathogenicity. {ECO:0000250|UniProtKB:P28240, ECO:0000269|PubMed:12622815}. |
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| Catalytic activity: | |
Reaction=D-threo-isocitrate = glyoxylate + succinate; Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031, ChEBI:CHEBI:36655; EC=4.1.3.1; Evidence={ECO:0000250|UniProtKB:P28240}; |
| Catalytic activity: | |
Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate; Xref=Rhea:RHEA:16809, ChEBI:CHEBI:15361, ChEBI:CHEBI:30031, ChEBI:CHEBI:57429; EC=4.1.3.30; Evidence={ECO:0000250|UniProtKB:P28240}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250|UniProtKB:P9WKK7}; |
| Pathway: | |
Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 1/2. |
| Subunit: | |
Homotetramer. {ECO:0000250|UniProtKB:P28240}. |
| Subcellular location: | |
Glyoxysome {ECO:0000250|UniProtKB:P28299}. |
| Disruption phenotype: | |
Leads to reduced virulence and a delay in the expression of rice blast symptoms. {ECO:0000269|PubMed:12622815}. |
| Similarity: | |
Belongs to the isocitrate lyase/PEP mutase superfamily. Isocitrate lyase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.